Bismark

Bismark Processing Report

Big_Data/Test_WGBS/trimmed/SRR9016928_sub500000_chr19_R1_clean.fastq.gz and Big_Data/Test_WGBS/trimmed/SRR9016928_sub500000_chr19_R2_clean.fastq.gz

Data processed at 14:04:35 on 2024-05-28


Alignment Stats

Sequence pairs analysed in total 286024
Paired-end alignments with a unique best hit 237856
Pairs without alignments under any condition 45934
Pairs that did not map uniquely 2234
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 6955907
Methylated C's in CpG context 35652
Methylated C's in CHG context 21214
Methylated C's in CHH context 53718
Methylated C's in Unknown context 92
Unmethylated C's in CpG context 337762
Unmethylated C's in CHG context 1695790
Unmethylated C's in CHH context 4811771
Unmethylated C's in Unknown context 851
Percentage methylation (CpG context) 9.5%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 118044 original top strand
CTOT 149 complementary to original top strand
CTOB 167 complementary to original bottom strand
OB 119496 original bottom strand

Deduplication

Alignments analysed 237856
Unique alignments 237654
Duplicates removed 202
Duplicated alignments were found at 191 different positions

Cytosine Methylation after Extraction

Total C's analysed 6793330
Methylated C's in CpG context 34291
Methylated C's in CHG context 19528
Methylated C's in CHH context 48921
Unmethylated C's in CpG context 330159
Unmethylated C's in CHG context 1657899
Unmethylated C's in CHH context 4702532
Percentage methylation (CpG context) 9.4%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.0%

M-Bias Plot