Bismark

Bismark Processing Report

Big_Data/Test_WGBS/trimmed/SRR9016927_sub500000_chr19_R1_clean.fastq.gz and Big_Data/Test_WGBS/trimmed/SRR9016927_sub500000_chr19_R2_clean.fastq.gz

Data processed at 14:04:35 on 2024-05-28


Alignment Stats

Sequence pairs analysed in total 283704
Paired-end alignments with a unique best hit 239274
Pairs without alignments under any condition 42415
Pairs that did not map uniquely 2015
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 7128860
Methylated C's in CpG context 66502
Methylated C's in CHG context 22641
Methylated C's in CHH context 47754
Methylated C's in Unknown context 69
Unmethylated C's in CpG context 324562
Unmethylated C's in CHG context 1745372
Unmethylated C's in CHH context 4922029
Unmethylated C's in Unknown context 804
Percentage methylation (CpG context) 17.0%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 119012 original top strand
CTOT 64 complementary to original top strand
CTOB 87 complementary to original bottom strand
OB 120111 original bottom strand

Deduplication

Alignments analysed 239274
Unique alignments 239101
Duplicates removed 173
Duplicated alignments were found at 167 different positions

Cytosine Methylation after Extraction

Total C's analysed 7000484
Methylated C's in CpG context 65207
Methylated C's in CHG context 21659
Methylated C's in CHH context 45197
Unmethylated C's in CpG context 318949
Unmethylated C's in CHG context 1714851
Unmethylated C's in CHH context 4834621
Percentage methylation (CpG context) 17.0%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 0.9%

M-Bias Plot