Bismark

Bismark Processing Report

Big_Data/Test_WGBS/trimmed/SRR9016926_sub500000_chr19_R1_clean.fastq.gz and Big_Data/Test_WGBS/trimmed/SRR9016926_sub500000_chr19_R2_clean.fastq.gz

Data processed at 14:04:33 on 2024-05-28


Alignment Stats

Sequence pairs analysed in total 284809
Paired-end alignments with a unique best hit 237003
Pairs without alignments under any condition 45582
Pairs that did not map uniquely 2224
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 6988271
Methylated C's in CpG context 259296
Methylated C's in CHG context 27227
Methylated C's in CHH context 64239
Methylated C's in Unknown context 72
Unmethylated C's in CpG context 106022
Unmethylated C's in CHG context 1702470
Unmethylated C's in CHH context 4829017
Unmethylated C's in Unknown context 765
Percentage methylation (CpG context) 71.0%
Percentage methylation (CHG context) 1.6%
Percentage methylation (CHH context) 1.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 118544 original top strand
CTOT 79 complementary to original top strand
CTOB 88 complementary to original bottom strand
OB 118292 original bottom strand

Deduplication

Alignments analysed 237003
Unique alignments 236814
Duplicates removed 189
Duplicated alignments were found at 183 different positions

Cytosine Methylation after Extraction

Total C's analysed 6800209
Methylated C's in CpG context 252143
Methylated C's in CHG context 26190
Methylated C's in CHH context 61340
Unmethylated C's in CpG context 103262
Unmethylated C's in CHG context 1657146
Unmethylated C's in CHH context 4700128
Percentage methylation (CpG context) 70.9%
Percentage methylation (CHG context) 1.6%
Percentage methylation (CHH context) 1.3%

M-Bias Plot