Bismark

Bismark Processing Report

Big_Data/Test_WGBS/trimmed/SRR11806589_sub500000_chr19_R1_clean.fastq.gz and Big_Data/Test_WGBS/trimmed/SRR11806589_sub500000_chr19_R2_clean.fastq.gz

Data processed at 14:04:38 on 2024-05-28


Alignment Stats

Sequence pairs analysed in total 297213
Paired-end alignments with a unique best hit 249687
Pairs without alignments under any condition 45460
Pairs that did not map uniquely 2066
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 7578890
Methylated C's in CpG context 53981
Methylated C's in CHG context 18123
Methylated C's in CHH context 50537
Methylated C's in Unknown context 85
Unmethylated C's in CpG context 358119
Unmethylated C's in CHG context 1852945
Unmethylated C's in CHH context 5245185
Unmethylated C's in Unknown context 1134
Percentage methylation (CpG context) 13.1%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 124683 original top strand
CTOT 403 complementary to original top strand
CTOB 355 complementary to original bottom strand
OB 124246 original bottom strand

Deduplication

Alignments analysed 249687
Unique alignments 249538
Duplicates removed 149
Duplicated alignments were found at 148 different positions

Cytosine Methylation after Extraction

Total C's analysed 7121539
Methylated C's in CpG context 50657
Methylated C's in CHG context 16918
Methylated C's in CHH context 46703
Unmethylated C's in CpG context 336933
Unmethylated C's in CHG context 1743035
Unmethylated C's in CHH context 4927293
Percentage methylation (CpG context) 13.1%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 0.9%

M-Bias Plot