Bismark

Bismark Processing Report

Big_Data/Test_WGBS/trimmed/SRR11806588_sub500000_chr19_R1_clean.fastq.gz and Big_Data/Test_WGBS/trimmed/SRR11806588_sub500000_chr19_R2_clean.fastq.gz

Data processed at 14:04:38 on 2024-05-28


Alignment Stats

Sequence pairs analysed in total 295267
Paired-end alignments with a unique best hit 249367
Pairs without alignments under any condition 43691
Pairs that did not map uniquely 2209
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 7894007
Methylated C's in CpG context 88475
Methylated C's in CHG context 72912
Methylated C's in CHH context 178365
Methylated C's in Unknown context 107
Unmethylated C's in CpG context 376554
Unmethylated C's in CHG context 1876650
Unmethylated C's in CHH context 5301051
Unmethylated C's in Unknown context 1507
Percentage methylation (CpG context) 19.0%
Percentage methylation (CHG context) 3.7%
Percentage methylation (CHH context) 3.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 124341 original top strand
CTOT 1120 complementary to original top strand
CTOB 983 complementary to original bottom strand
OB 122923 original bottom strand

Deduplication

Alignments analysed 249367
Unique alignments 249187
Duplicates removed 180
Duplicated alignments were found at 175 different positions

Cytosine Methylation after Extraction

Total C's analysed 7339831
Methylated C's in CpG context 82153
Methylated C's in CHG context 67691
Methylated C's in CHH context 165309
Unmethylated C's in CpG context 349950
Unmethylated C's in CHG context 1746774
Unmethylated C's in CHH context 4927954
Percentage methylation (CpG context) 19.0%
Percentage methylation (CHG context) 3.7%
Percentage methylation (CHH context) 3.2%

M-Bias Plot