Bismark

Bismark Processing Report

Big_Data/Test_WGBS/trimmed/SRR11806587_sub500000_chr19_R1_clean.fastq.gz and Big_Data/Test_WGBS/trimmed/SRR11806587_sub500000_chr19_R2_clean.fastq.gz

Data processed at 14:04:12 on 2024-05-28


Alignment Stats

Sequence pairs analysed in total 296117
Paired-end alignments with a unique best hit 249292
Pairs without alignments under any condition 44621
Pairs that did not map uniquely 2204
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 7852521
Methylated C's in CpG context 303011
Methylated C's in CHG context 56559
Methylated C's in CHH context 135271
Methylated C's in Unknown context 110
Unmethylated C's in CpG context 146320
Unmethylated C's in CHG context 1895461
Unmethylated C's in CHH context 5315899
Unmethylated C's in Unknown context 1342
Percentage methylation (CpG context) 67.4%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 2.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 123925 original top strand
CTOT 1147 complementary to original top strand
CTOB 1064 complementary to original bottom strand
OB 123156 original bottom strand

Deduplication

Alignments analysed 249292
Unique alignments 249130
Duplicates removed 162
Duplicated alignments were found at 158 different positions

Cytosine Methylation after Extraction

Total C's analysed 7328172
Methylated C's in CpG context 282948
Methylated C's in CHG context 52661
Methylated C's in CHH context 125904
Unmethylated C's in CpG context 136480
Unmethylated C's in CHG context 1770538
Unmethylated C's in CHH context 4959641
Percentage methylation (CpG context) 67.5%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 2.5%

M-Bias Plot