Alignment Stats
| Sequence pairs analysed in total |
296117 |
| Paired-end alignments with a unique best hit |
249292 |
| Pairs without alignments under any condition |
44621 |
| Pairs that did not map uniquely |
2204 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
7852521 |
| Methylated C's in CpG context |
303011 |
| Methylated C's in CHG context |
56559 |
| Methylated C's in CHH context |
135271 |
| Methylated C's in Unknown context |
110 |
| Unmethylated C's in CpG context |
146320 |
| Unmethylated C's in CHG context |
1895461 |
| Unmethylated C's in CHH context |
5315899 |
| Unmethylated C's in Unknown context |
1342 |
| Percentage methylation (CpG context) |
67.4% |
| Percentage methylation (CHG context) |
2.9% |
| Percentage methylation (CHH context) |
2.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
123925 |
original top strand |
| CTOT |
1147 |
complementary to original top strand |
| CTOB |
1064 |
complementary to original bottom strand |
| OB |
123156 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
249292 |
| Unique alignments |
249130 |
| Duplicates removed |
162 |
| Duplicated alignments were found at 158 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
7328172 |
| Methylated C's in CpG context |
282948 |
| Methylated C's in CHG context |
52661 |
| Methylated C's in CHH context |
125904 |
| Unmethylated C's in CpG context |
136480 |
| Unmethylated C's in CHG context |
1770538 |
| Unmethylated C's in CHH context |
4959641 |
| Percentage methylation (CpG context) |
67.5% |
| Percentage methylation (CHG context) |
2.9% |
| Percentage methylation (CHH context) |
2.5% |
|
|
M-Bias Plot